| Preliminary Observations | Page 5 |
| Chap. I. - On the requirements for producing the desired result | Page 6 |
| Chap. II. - The Lens and its Combinations:- | |
| Various forms and focal lengths | Page 7 |
| Mounting and adjustments | Page 8 |
| Chap. III. - Methods of Employing the Instrument:- | |
| By open daylight | Page 10 |
| By sunshine | Page 12 |
| By artificial light | Page 14 |
| Chap. IV. - On some matters of choice in the Instrument and its use | Page 15 |
| Chap. V. - On the Conditions required in the Negative:- | |
| Methods of obtaining them | Page 16 |
| Chap. VI. - The Copies:- | |
| Collodion and its preparation for microscopic use | Page 18 |
| Mode of Development | Page 20 |
| Fixing agent, and how to use it | Page 21 |
| Chap. VII. - Mounting the Copies:- | |
| The preparation of the material employed and how to use it | Page 22 |
| Conclusion | Page 23 |
| Appendix. - On certain subsidiary methods, and practical applications of the art | Page 25 |