Stars of the Marshes
by Wim van Egmond


The last Friday of May this year the Dutch Microscopy Club NVVM had an excursion to the fens of Oisterwijk. Host of this trip was one of our fellow members Frans Roefs who lives near the location. He is an amateur microscopist like most of our members, but by chance he became involved in research carried out by Natuurmonumenten, the owner of these fens.Their aim is to monitor the different small lakes and find out how to restore the original ecosystem as well as possible. Because Frans is studying the lakes and knows the area very well he was the perfect guide. He could show us the most interesting spots.

That day we had a good catch with several kinds of interesting algae. Most prominent were the desmids. There were all kinds of Closterium, Cosmarium, Staurastrum and a few Micrasterias species in our finds. For me this was the right occasion to make sketches of the several species of Staurastrum.



About the images.

Staurastrum is a particularly interesting organism to make drawings of because it's so tiny. That makes it very difficult to photograph. To see the fine details you should use a 100x immersion lens. The image then presented has such shallow depth of field that it's not easy to see the three dimensional shape. And the shapes of these organisms are most fascinating.

 

 



So I started sketching them from different angles. When I had the three dimensional shape in my mind I started to make the final drawing. I'm afraid I don't use the traditional method of taking exact measurements. My aim is to make an impression of what I think the organisms look like.



I made a group portait of Staurastrum and related genera. What I found most difficult was showing both the ornamentation as well as the inner structure. The cell wall is covered with rows of spikes or knobs. All in a regular pattern. The inner structure contains two pyrenoids, in each semi-cell. Between them, in the actual center of the organism I could sometimes see the nucleus. The chloroplast fills the rest of the cell. Because of the form of Staurastrum the chloroplast is split in three parts, each part appears to have two halves. In the center of the drawing there is a Staurastrum still attached after cell-division. Some species are not symmetrical, one half being slightly rotated to the other. After cell division this results in the two cells lying in the same direction.



Peridinium

Working with the 100x immersion lens can be a bit messy but the result is that you can see many details. I examined a small dinoflagellate found in the same water as the Staurastrum. When you look very carefully and adjust your microscope as good as possible you can see the two flagella (one moving through a groove) and the structure of the plates of the cell wall. The possibilities of the light microscope are not to be underestimated. It may be more difficult to see the three dimensional shape than it is with a electron microscope. But what we see is alive and we can look right through it.

My next article will be about three dimensional photography through an ordinary light microscope and how to make a stereoscope from a binocular without spending a fortune.

Note: We only used the genus names. Even with the help of experts there remained several uncertainties.

The author Comments to the author sent via our contacts page quoting page url plus : ('wegmond','')">Wim van Egmond would welcome comments.



Editor's notes:
The desmid illustrations are derived from the original group portrait.
Click here to view a scan of this original drawing.

The Editor and author would like to thank Bill Ells for useful comments on this article and the desmid identification.

The images are the copyright of the author, and should not be either redistributed or used commercially without seeking the permission of the author via Jan Parmentier.



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