A low cost
set-up for capturing digital images
by David Young, Iowa City, USA
One of the most important messages Micscape tries to put across is that you can have fun with microscopy and macroscopy using the very simplest equipment. David Young shares his experiments with kit bought for a few dollars at yard sales.
Hello! I am from Iowa City and I just wanted to share with you some of the details of my personal set-up for taking digital pictures through the microscope. The equipment is dirt cheap and fairly simple.
I use a second-hand microscope bought at a yard sale for five dollars, which I have now repaired (shown right). The eyepiece, objective lenses and part of the stage assembly have been cobbled together from parts of other microscopes etc. It had been clearly neglected but once it was cleaned and oiled, the mechanics - especially the coarse and fine focus, proved to be one of the best I have encountered. It's a pity it didn't come with its original lenses but it works like a charm and I can even make out individual bacteria cells with it. (By the way, can anyone identify when this particular instrument by Bausch and Lomb was made? I sure would like to know.)
For the microscope camera I use a 'JamC@m 2' made by KBGear Inc. which is a digital stills camera providing up to 640 x 480 resolution (shown in use right). It costs about $80 - $100 depending on source. The camera is fixed focus (focusing from about one foot to infinity) and is simply held over the microscope eyepiece by hand. The lens mount is recessed slightly into the camera casing which fits over the smaller eyepiece quite easily. It doesn't even have a flash and is similar to those disposable film cameras in usage i.e. just point and shoot. The entire set-up - microscope plus camera and the assortment of lenses etc. used to restore the microscope cost me about $100. It's interesting to compare this to the IntelPlay QX3 toy video microscope which sells at a similar price.
Unfortunately I only have two cameras available to me: a 'Webcam 2' (Creative Labs Inc.) which was used to take the photo' of my 'scope and camera, and the 'Jamcam 2' used to take the photo's through the microscope. The webcam only has a resolution of 320 x 240 pixels so the image above is the best achievable.
Pictures taken with the microscope set-up are a bit on the blurry side, but I have improved the results using the sharpening filter in 'Paint Shop Pro'. Also the color balance is off but that appears to be a result of the low light used i.e. an ordinary desk lamp. The microscope has no iris diaphragm or condenser to adjust the lighting, but you can still make out many of the details in subjects like pond life. Even the thin hair-like stalks of the bell animal (Vorticella?) shown below when photographed alive are visible.
When using the 'JamC@m 2' over the microscope I first focus the microscope by eye then place the camera over it, steady the camera by hand and press the shutter button. Its very simple - no special stands, camera mounts etc. used. It is slightly tricky to line up the camera and on average I mess up one shot out of eight.
I use an IBM model 750 computer with a P-90 processor for image processing, which of course is very outdated but still very adequate ... and thats it!
Comments to the author Comments to the author sent via our contacts page quoting page url plus : ('dyoung','')">David Young welcomed.
Image gallery by the author.
Here is a selection of ant pictures. I keep ants as a hobby and have a colony of red ants (Myrmica species) which I am raising from queens collected last year. I also have a colony of Aphaenogaster species. These photos were taken using only a 'Webcam 2' without additional lenses. For the really close-up shots I removed a plastic focus knob which prevented focusing closer than five centimeters.
Eye of a red ant (Myrmica species).
Antennae of a red ant (Myrmica species).
Image right, a sack spider.
Published in the February 2000 edition of Micscape Magazine.
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