By Ian Walker. U.K.









All the following pictures were taken on a Nikon Coolpix 4500 digicam together with a Zeiss microscope and objectives. At present I'm still trying different settings and techniques using the Nikon for microscopy and so some of the following pictures could be better!
 

*Note that most of the pictures have been post edited with Photoshop Elements® to improve contrast and definition. The magnification shown for each image refers to those seen through the eyepiece not taking into account the final image size.*
 


Below are the slides, in order, of the pictures in the article.
 


On the left below, H.R.S. Williams: mounted in 1922, part of his slide showing six selected Coscinodiscus, Actinocyclus and Stictodiscus diatoms.
On the right is from Watson & Sons Ltd, one of three nicely arranged Actinocyclus subtilis.


both taken at X630 in black and white using phase contrast.



 
 

Unamed mount: mounted in 1965 possibly by R. Gosden, of Auliscus gigas from Palos Verdes 6A, California.
 


 

On the left X630 using phase contrast in black and white, on the right X400 phase contrast with blue filter in colour.



 
 

A very nice 'classic' slide by Richard Suter showing various Isthmia nervosa.
 


 

X24 by top lighting, this shot was achieved with a 'MINI MAGLITE'® torch and a very cheap unamed achromatic 4X objective!



 
 

A. C. Cole: two beautifully mounted Aulacodiscus diatoms from Peru, the mount is still incredibly clear and free of defects after many years. This shot required very little editing due to its clarity, except for modest enhancement after resizing.
 










 
 

R. Gosden: mounted 1963 in Naphrax of a Navicula from Newport Beach, California, U.S.A. This slide looks excellent in bright field as well.
 

X630 Phase Contrast.



 
 

H.R.S. Williams: mounted 1922 in Styrax, this is part of his slide of five nicely arranged diatoms.
 

On the left, Arachnoidiscus and on the right the central portion in colour at X250 using phase contrast with a blue filter.



 
 

Uknown mounter: mounted in 1922, these photos don't do this slide justice, four beautifully mounted Stictodiscus buryanus from a fossil deposit, Haiti, again looking good after 80 years.


 

On the left X100 bright field in black and white and on the right, X250 showing the central diatom in phase contrast [at a different focus point] with a blue filter.












 
 

Fred Reed: mounted in 1957, this shows a 'brackish' diatom from Whangamarino, New Zealand, now recognized as an important wetland site for a large number of rare and endemic plants and animals.
 


 

On the left, the Whangamarino diatom in black and white and on the right at X250 using phase contrast [at a different focus point] with blue filter.
 
 
 

the end.

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