A Weird Wee Beastie: Trichoplax adhaerens

by Richard L. Howey, Wyoming, US

 


Living nearly 1000 miles from the nearest seashore, I have little access to marine specimens. Last year, through the generosity of the proprietors of a local pet shop, I procured detritus samples from their marine aquaria (see footnote 1). I was interested primarily in looking for marine protozoa and was gratified to find a number of fascinating and elegant types of protozoa. I also noticed what appeared to be a number of pinkish "deposits" which appeared to be some kind of odd inorganic, undistinguished something or other. For several weeks, I was too fascinated with the marine protozoa to take any further notice. Then, one afternoon, I observed one of these "deposits" moving. I was looking at sample in a small culture dish using a binocular zoom dissecting microscope set on 20x. I immediately zoomed to 40x and observed an amoeboid-like movement. Initially, I was convinced that I had found a large and rather strange marine amoeba with a pinkish tinge.

An artist's impression of Trichoplax adhaerens prepared by Wim van Egmond using Adobe Photoshop.
Visit Wim's homepage


As often happens, I was interrupted and didn't get back to observing this sample for over a week. Upon resuming my observations, I found something very odd indeed. The organisms now looked rather like plasmodial slime molds. They were "strung out" in unusual configurations which appeared to be an amoeba with a thin protoplasmic bridge connecting to another amoeba-like form with yet another thin protoplasmic bridge connecting to yet another amoeba-like form; yet all of this comprised a single organism. I had never seen anything behave in such a bizarre manner. Stranger still, was that there were folds along some of the edges and sometimes within one or more of the masses of the amoeba-like parts. These latter folds seem to project upwards. I took a transfer pipet and tried to move some of the organisms to a slide to look at them more closely. Unfortunately, this caused the organisms to come apart and they were very difficult to get into the pipets, since they seemed to be glued to the bottom of the culture dish, thus earning their species name, adhaerens.

I had taken an old aquarium and thrown in some of the original samples (which contained some filamentous algal forms) and hoped that this would provide a plentiful supply of these "weird wee beasties." In a few weeks, it did and I was able to take samples from the sides of the aquarium and transfer them to slides and small dishes with minimal damage to the organisms. However, the first attempts to use slides were not very successful. The surface tension of the drop would tear the organisms apart. I finally decided to fit up one of my microscopes with some old, inexpensive objectives and use them as water immersion lenses. This allowed me to set the culture dish on the stage and observe the organisms without transferring or disturbing them. This led to a great surprise—under 400x, I observed that the surface of the creatures was covered with flagella! A flagellated amoeba! I had never heard of such a thing and was fairly certain that this was not a protozoan at all (see footnote 2).

With much fussing and fidgeting, I was able to get some video-tape of the organism through the microscope. I then took the video-tape up to three of my colleagues in the Zoology department. Two of them are cell-biologists (one of whom is a specialist in protozoa) and the third is an invertebrate zoologist who is also a specialist in slime molds. One of them was initially convinced that it was indeed a large amoeba, another conjectured that it might be an early stage of a sponge or a coelenterate or more likely, in his view, a very primitive non-segmented worm. So, no help from these guys. Off and on for the next six or eight weeks, I browsed in the library in books on the lower invertebrates, protozoa, algae, and everything I could put my hands on that might help me identify this creature. Then one evening I came across a drawing that looked very much like my beastie. It was identified as Trichoplax adhaerens and the drawing also looked like a large amoeba, but the description reported that Trichoplax is the most primitive multi-cellular animal known and has the smallest amount of DNA of any animal ever sequenced. There are reports of another organism in this group, Treptoplax reptans, but most of those who know this animal think that the second organism described is really Trichoplax. In any case, this organism has a whole phylum to itself, viz., Placozoa.

I was almost certain that this was the right organism, but I still was puzzled about the odd "strung out" forms which I had observed and video-taped. So, back to the library. Now that I had the name, I could check the literature and find other articles which might confirm my strong intuition. Eventually I found thirty-four articles, a book chapter, and three short films on Trichoplax. (Let me say before proceeding with my ramblings, that I did indeed find an article on the "strung out" forms and was confirmed in my opinion that the organism I had was indeed Trichoplax.) So, now permit me to say something about the discovery and history of the research on this wonderfully odd animal.

Trichoplax was first described by F.E. Schulze, a German scientist, in 1883. For a while it was thought to be the planula stage of a hydromedusan and that theory has been resurrected on at least two occasions since Schulze. At first there was a good bit of excitement, but then gradually the view that it was a planula was accepted and Trichoplax was largely forgotten. This planula-view was published in an article in 1912. The next article which I have located appears fifty-four years later in 1966 again in Germany and then in the 1971, there appeared the first of many important and definitive studies on Trichoplax published by Karl Grell, the distinguished protozoologist and director of the Institute for Zoology at Tübingen. Grell was also responsible for the three short films on various aspects of Trichoplax.

Trichoplax is an interesting organism to study, because it is one of those "missing links" that provides some hints about the evolution of some of the metazoa. It has only three cell layers and purportedly only four different kinds of cells. However, it raises interesting questions, some of which are still not completely answered. How it feeds is still something of a perplexity. It seems that it absorbs its nutrition through the ventral surface and probably feeds on algae. I have observed it draped on pieces of a filamentous alga. In its immediate vicinity, the contents of the algal cells appear to have been digested leaving only the cellulose envelope of the filament.

The extraordinary shapes which it can assume are apparently accounted for my the middle layer of "fibrous" cells which seem to provide both a degree of support and also allow for its radical alterations in form. A student whom I assisted with a project on Trichoplax was able to show that when the cells are disassociated; they will regroup, but not necessarily according to the organism from which they came. He used two vital stains—red and toluidine blue. When the cells reaggregated some of the specimens contained cells that were red and others that were blue.

Trichoplax is an organism that requires a good deal of patience to study, but it well worth the time and effort. Apparently not a great deal is known about the ecology of this organism. Grell got his samples from algal collections that a colleague of his brought back from the Red Sea. The samples which I got from the pet shop aquaria are apparently also from tropical locations. However, Trichoplax seems quite adaptable judging from the neglect under which it thrived in my cultures, so it may well occur in areas in which the water is considerable colder. I suspect that its distribution may be fairly wide-spread and than it simply has not been looked for in very many environments. Interestingly most of the reports, other than Grell's, have come from investigators who happened to notice it in aquaria. Once it is established in a culture, it is quite noticeable as small white clumps or stringy blobs on the side of the aquarium. I have taken to using small fish bowls that hold about a gallon of water and when the Trichoplax become abundant in one jar, I start two or three others to ensure a constant supply. I keep the jars in a window where there is abundant indirect light. It is important to make sure that algae will also grow in the culture jars and I usually add 6 or 8 boiled wheat grains to provide food for small organisms that the Trichoplax may or may not feed on. I use standard marine salts which I purchase and make up a standard solution with artesian or distilled water. Trichoplax seems able to tolerate a range of salinity and so the proportions don't seem critical. I do monitor the specific gravity of the solution and try to keep it fairly constant.

When Trichoplax become very abundant in a jar, their population, for some unknown reasons, starts to decline. For this reason, it is important to try to keep several cultures going at once with periodic sub-culturing. When the organisms are healthy, they have a light rose color. The source of this pigmentation is unknown. Trichoplax reproduces in three ways: 1) by binary fission (and this probably takes place when the organisms are "strung out", 2) by budding, i.e., a spherical body forms on the dorsal surface and eventually separates off, and 3) Grell reports the existence of ova, although no sperm cells have been identified.

Another fascinating aspect of this creature is its ability to regenerate. As I mentioned above, the organism is often damaged in the process of transferring it to a culture dish for examination. However, this damage can be repaired and the resulting smaller pieces continue to behave as before. Obviously, there is a minimum amount of material which must be available for this repair process to take place. In some instances, the organism seems to just dissipate, but in other instances, after some days, one may notice a number of very small Trichoplax in the culture dish.

Another oddity of Trichoplax is that while it is frequently found either along the bottom or the sides of dishes or aquaria, I have found some cultures in which significant numbers of the organism can be found floating on the surface of the water.

Finally, Trichoplax is an exciting organism to observe, because it has an odd set of both structural and behavioral characteristics. When it is "strung out", one can observe protoplasmic streaming in the "bridges" between the main masses. There are strange "folds" which develop along the edges and the dorsal surface of creature whose function is largely unknown. Along the edges of the organism are very distinct "shiny spheres". To observe the amoeboid movement and, at the same time, see the surface covered with flagella is a remarkable phenomenon. Trichoplax is truly one of nature's oddities.

Footnotes

1) I want to thank Rick and Jennie Lawrence of Peaceable Kingdom Pet Shop in Laramie, Wyoming for their generosity and patience in supplying me samples. Return to article.
2) Actually there are some protozoa that have both amoeboid and flagellated forms, but usually only one or two flagella and the organisms are quite small. Return to article.

Editor's notes:

Comments to author Comments to the author sent via our contacts page quoting page url plus : ('rhowey','')">Richard Howey are welcomed.

First published off-line in the Manchester Microscopical and Natural History Society newsletter Micro Miscellanea.

 

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