image 01
 No formalin, no mercury fixatives.
(Part 2)
WALTER  DIONI                       Durango (Dgo) México
The title image is a very young mayfly (Ephemeroptera) larva, fixed in 70% alcohol and mounted in PVA-Glycerol. Imaged with the 4x planachromatic objective on a National Optical microscope with a COL (circular oblique illumination) filter. The field image width was 3.4 mm. This and all the other images in the article were captured with the digital camera integrated onto the microscope.

Alcohol 70%
image002
image 003
Chydoridae, fixed with 70% alcohol, wet mounted in 1:3 glycerin-water. Three image mosaic. Captured with the x10 objective. Half reduced. Background somewhat cropped.
Young Culex mosquito larva. Fixed with 70% alcohol many months ago. Mounted in  PVA-Glycerol. Captured with the 4x objective. Width of the field of view 3.4 mm. COL filter.
Sample (ml)
5
10
15  
  20
25
Alcohol 96 (ml)
13.5
27
40
54
67
Total volume (ml) 
18.5
37
55
74
92
Alcohol 90 (ml)
17.5
35
52.5
70
87.5
Total volume (ml)
22.5
45
67.5
90
112.5

Alcohol 96%..........................95 ml
Acetic acid..............................5 ml

image 004
image 005
A chlorophycean algae, with a gelatinous envelope of a species unknown to me. x40 objective. Somewhat scaled up. Very cropped image
Two specimens of Euglena deses, objective x40. 70% alcohol. Mounted in the same. Captured inside the empty valves of a cladoceran. Cropped image.
 
IMAGE 006
IMAGE 007
Diatom, the day after being fixed with hot water. x40
Euglena deses. x100 0I. Hot water.

Cropped  pictures from wet mounts sealed with nail polish.
image008
image 009
image 010
Epiphanes, captured x40, mosaic of two pictures
Mytilina, captured x40, amalgamated with Combine Z
Cyclopid copepod, captured x40. Mosaic of 10 images. Original is 1028 x2000 pixels.
I have lost the original pictures of these two beautiful rotifers.So I present only the two icons that remained.
Click over the image to see a 55% reduction of the original
amoeba
An amoeba at 100x killed by the boiling water!!
 
Fixative of Rhode ("Lugol's solution")


image 012

image 013

image 014
Euglena prob. oxyuris, x100 OI
Collage of microflagellates x100 OI, taken from several pictures. Click the image to see a labeled copy
Phacus pyrum, x100 OI
image 015
Chaetonotoidea gastrotrich x100 OI. Click the image to see a labeled and bigger copy. This is not a normal image of a fixed gastrotrich. Normally they roll-up and become almost illegible. This one was very compliant. This is a central optical section that shows most of the anatomy details of these elusive species.
 
microplancton
A miscellanea of decanted bacteria and microphytoplakton.
Good hunting!



Comments to the author, Walter Dioni , are welcomed.


 

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