Amoeba proteus in different spot-lights

Text and images by Steve Durr

Illumination and contrast techniques for the light microscope


These pages introduce several illumination and contrast techniques available for the light microscope. To show the difference between the illuminations one particular organism was photographed in bright field, dark field, phase contrast and Nomarski (differential interference contrast or D.I.C.)

The protozoan Amoeba proteus, which is about 750 micron (0.75 millimetre) in length, can sometimes be found in clear well-oxygenated waters crawling around the detritus at the bottom of the pond.

Click the thumbnails to enlarge the images and read more about these techniques


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