An illustration of the light path in darkfield illumination
  
by Wim van Egmond
 
 

This illustration shows the way the darkfield patch stop blocks the central part of the light beam. The subject on the slide is illuminated while the light rays do not enter the objective. Only the light refracted by the subject enters the objective.

This way the subject will appear light on a dark background.
 
 

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Darkfield, oblique and bright field illumination
  


Rheinberg illumination
 
All comments to the author Wim van Egmond are welcomed.

Visit Wims home page for links to his many web pages on microscopy

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