An illustration of the light path in darkfield illumination
by Wim van Egmond

This illustration shows the way the darkfield patch stop blocks the central part of the light beam. The subject on the slide is illuminated while the light rays do not enter the objective. Only the light refracted by the subject enters the objective.

This way the subject will appear light on a dark background.

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Darkfield, oblique and bright field illumination

Rheinberg illumination
All comments to the author Wim van Egmond are welcomed.

Visit Wims home page for links to his many web pages on microscopy

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Published in the April 2002 edition of Micscape Magazine.

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