Technical details for Micscape article HOMAGE to the ONION SKIN

with some additions

Walter Dioni, Mexico


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Anopheles mosquito larvae, malaria parasite vector. 4x Objective. 3 pictures stitched with PhotoPaint.- COL-D
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Daphnia - x10 objective
Cheek epithelium cells - 100x obj.- cropped from the 640 x 480 original - contrast raised and lowered intensity for better displaying the cells.
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Mosquito larvae - 4x objective
Mosquito larvae - 4x objective- COL-D1

This is not a science, it is an art. The only guarantee of success lies in experimentation. But these tools are those which drives the amateur microscope with a power very close to that of much more expensive professional microscopes. If you do not have too much money you need these small tools, which give such fine and beautiful images.

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Daphnia, alive, 4x, 12.5 DFS

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Daphnia, 4x obj. 10 mm  centred DFS
The same, 15 mm DFS off centre
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Cheek cells, 100x obj, - MOI

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Daphnia head, 40x obj. - ObI - 10 mm marginal clear disk on 30.5 opaque stop.
Cheek cells, 100x obj. half reduced
 Mathias wedge.

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Onion skin - 10x obj.- RhF - blue center - red annulus
Anabaena - 10x obj. - RhF - blue center - clear annulus

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Anopheles egg - 10x obj. - blue center -yellow annulus

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Larvae of Ephemeroptera -4x obj.
The same - COL -J

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The same -COL-C

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Algae (Zygnema and Spyrogira) - 40 x obj. - COL-J
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 Detail, mosquito larvae - 10 x obj - COL-C

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Daphnia embryos - 10x obj - COL-D1
3 mosquito larvae segments - 10x obj - COL-D2

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Anabaena - 40x obj. - COL-D3
Anabaena -100 x obj. - COL-D3
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Micro-Oligochaete - 100x obj. -COL-D1 + RhFQ
Larvae of Ephemeroptera - 4x -COL-D3
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Although it appears to me only an anecdotal and marginal application, the relief can be distinguished even in histological preparations coloured with HE and mounted in Balsam, with a thickness of barely 5 microns, and various tissues (especially the connective ones) sometimes gives different brightnesses and colours.

Critical illumination - COL-D1 - COL-D3

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 Euchlanis - a rotifer
Prorodon - a ciliate
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An algae  - Spyrogira
A turbellarian - Macrostomum

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Compound flower of Gazania sp. ( x 0.5 of natural size aprox.) At right one of the true flowers from the circle pointed by the arrow . Original is 700 x 827 pixels here reduced to 350 x 413.
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The first 3 pictures were treated by a method to produce 3D images from flat ones. The last one was taken using the Red-Blue filter of Wim van Egmond and shows the real position of the pollen in the slide. The original of third picture taken with the COL-D3 stop.

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Comments to the author, Walter Dioni, are welcomed.



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